Role of Glutathione Conjugation in 1-Bromobutane-induced Immunotoxicity in Mice

Title
Role of Glutathione Conjugation in 1-Bromobutane-induced Immunotoxicity in Mice
Author(s)
이상규이동주전태원고규섭유진우하현우강미정강원구[강원구]김상겸[김상겸]정태천
Keywords
1-Bromobutane; Glutathione; Conjugation; Immunotoxicity; In vivo
Issue Date
201006
Publisher
한국독성학회
Citation
한국독성학회지, v.26, no.2, pp.101 - 108
Abstract
Halogenated organic compounds, such as 1-bromobutane (1-BB), have been used as cleaning agents, agents for chemical syntheses or extraction solvents in workplace. In the present study, immunotoxic effects of 1-BB and its conjugation with glutathione (GSH) were investigated in female BALB/c mice. Animals were treated orally with 1-BB at 375, 750 and 1500 mg/kg in corn oil once for dose response or treated orally with 1-BB at 1500 mg/kg for 6, 12, 24 and 48 hr for time course. S-Butyl GSH was identified in spleen by liquid chromatography-electrospray ionization tandem mass spectrometry. Splenic GSH levels were significantly reduced by single treatment with 1-BB. S-Butyl GSH conjugates were detected in spleen from 6 hr after treatment. Oral 1-BB significantly suppressed the antibody response to a T-dependent antigen and the production of splenic intracellular interlukin-2 in response to Con A. Our present results suggest that 1-BB could cause immunotoxicity as well as reduction of splenic GSH content, due to the formation of GSH conjugates in mice. The present results would be useful to understand molecular toxic mechanism of low molecular weight haloalkanes and to develop biological markers for exposure to haloalkanes.
URI
http://hdl.handle.net/YU.REPOSITORY/22305
ISSN
1976-8257
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약학대학 > 약학부 > Articles
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