Gene Expression Profiles of Uterine Normal Myometrium and Leiomyoma and Their Estrogen Responsiveness In Vitro

Title
Gene Expression Profiles of Uterine Normal Myometrium and Leiomyoma and Their Estrogen Responsiveness In Vitro
Author(s)
최인호이은주프라티[프라티]이동목조경현김극준[김극준]배영경김미진이기호[이기호]김항진[김항진]송건호[송건호]전상식[전상식]
Keywords
MESSENGER-RNA EXPRESSION; SMOOTH-MUSCLE-CELLS; EXTRACELLULAR-MATRIX; BREAST-CANCER; FIBROIDS; BINDING; TROPOMYOSIN; RECEPTORS; CULTURE
Issue Date
201007
Publisher
KOREAN SOCIETY PATHOLOGISTS
Citation
KOREAN JOURNAL OF PATHOLOGY, v.44, no.3, pp.272 - 283
Abstract
Background : Uterine leiomyomas are common benign smooth muscle tumors among the reproductive aged-women. The research has been aimed to identify the differentially expressed genes between normal myometrium and leiomyoma and to investigate the effects of E2 on their expression. Methods : Gene microarray analysis was performed to identify the differentially expressed genes between normal myomerium and leiomyoma. The data was confirmed at protein level by tissue microarray. Results : Gene microarray analysis revealed 792 upregulated genes in leiomyoma. Four genes (tropomyosin 4 [TPM4], collagen, type IV, alpha 2 [COL4 alpha 2], insulin-like growth factor binding protein 5 [IGFBP5], tripartite motif-containing 28 [TRIM28]) showed the most dramatic upregulation in all leiomyoma samples. Tissue microarray analyses of 262 sample pairs showed significantly elevated expression of TPM4, IGFBP5, estrogen receptor-alpha, and progesterone receptor (PR) protein in leiomyoma from the patients in their forties, COL4 alpha 2 in the forties and fifties age-groups, and TRIM28 in the thirties age-group. PR, insulin-like growth factor 1 (IGF-1), IGF-1 receptor (IGF-1R) and IGFBP5 were induced by E2 in in vitro culture of tissue explants from which cells migrated throughout the plate. Among these, PR, IGF-1, IGFBP5 genes showed higher expression in tissue compared to cells-derived from tissue in leiomyoma and IGF-1R in leiomyoma cell. Conclusions : This observation implies the importance of the whole tissue context including the cells-derived from tissue in the research for the understanding of molecular mechanism of leiomyoma. Here, we report higher expression of TRIM28 in leiomyoma for the first time and identify E2-responsive genes that may have important roles in leiomyoma development.
URI
http://hdl.handle.net/YU.REPOSITORY/22196http://dx.doi.org/10.4132/KoreanJPathol.2010.44.3.272
ISSN
1738-1843
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생명공학부 > 생명공학부 > Articles
의과대학 > 병리학교실 > Articles
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