Identification and analysis of dominant negative mutants of RAIDD and PIDD
- Identification and analysis of dominant negative mutants of RAIDD and PIDD
- 박현호; 장태호; 배주영; 박옥경; 김지회; 조경현; 전주홍[전주홍]
- DOMAIN-CONTAINING PROTEIN; PROGRAMMED CELL-DEATH; INFLAMMATORY CASPASES; CRYSTAL-STRUCTURE; IMMUNE-SYSTEM; DNA-DAMAGE; KINASE RIP; APOPTOSIS; ACTIVATION; COMPLEX
- Issue Date
- ELSEVIER SCIENCE BV
- BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, v.1804, no.7, pp.1557 - 1563
- Caspases are cysteine proteases that are essential during the initiation and execution of apoptosis and inflammation. The formation of large oligomeric protein complexes is critical to the activation of caspases in apoptotic and inflammatory signaling pathways. These oligomeric protein complexes function as a platform to recruit caspases, which leads to caspase activation via a proximity-induced mechanism. One well-known oligomeric caspase-activating complex is the PIDDosome for caspase-2 activation, which is composed of 3 protein components, PIDD, RAIDD and Caspase-2. Despite the significant role that caspase-2 activated by PIDDosome plays during genotoxic stress-induced apoptosis, the oligomerization mechanism and the method by which the caspase-activating process is mediated by the formation of PIDDosome is currently not well understood. Here, we show that the assembly mechanism of the core of PIDDosome is time-dependent and salt concentration-dependent. In addition, we demonstrate that point mutations on RAIDD (R147E) and on PLOD (Y814A) exert a dominant negative effect on the formation of the PIDDosome, and that this effect cannot be applied after the PIDDosome has been formed. Crown Copyright (C) 2010 Published by Elsevier B.V. All rights reserved.
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